The Tennis Star has not competed since winning the Australian Open in January but has posted several videos to social media showing her hitting balls during her pregnancy.
Congratulations have been pouring in from sports stars and celebrities.
News of the birth came as her sister Venus prepared to go out on court at the US Open.
“Obviously I’m super-excited,” Venus said. “Words can’t describe it.”
The couple are yet to confirm the birth themselves but Serena’s coach, Patrick Mouratoglou, tweeted: “I am so happy for you and I feel your emotion.”
He added: “Btw … I wish you a speedy recovery… we have a lot of work ahead of us.”
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‘Hope she doesn’t play tennis’
Serena admitted she had revealed her pregnancy to the world in April by accident, after mistakenly uploading a photograph on Snapchat.
She won the Australian Open title this January while newly pregnant, and in an article in Vogue last month she said she wanted to defend her title.
“It’s the most outrageous plan,” she said. “I just want to put that out there. That’s, like, three months after I give birth.”
In June she appeared in a nude cover photo for Vanity Fair, saying: “I don’t know what to do with a baby.”
The news has delighted the tennis world, with Rafa Nadal among the first to tweet his congratulations.
At a news conference, Garbine Muguruza joked; “A baby girl? Well, I hope she doesn’t play tennis,” Reuters reported.
Singer Beyonce posted a portrait of a pregnant Williams on Instagram, with the message: “Congratulations Serena!”
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